Assessment of escape from X chromosome inactivation and gene expression in single human immune cells

Summary: X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the s...

ver descrição completa

Detalhes bibliográficos

Principais autores:	Sven Hendrik Hagen, Jana Hennesen, Marcus Altfeld
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2021-09-01
coleção:	STAR Protocols
Assuntos:	Flow Cytometry/Mass Cytometry Immunology Molecular Biology Gene Expression
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166721003488
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!