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Isolating the Mutator Transposable Element Insertional Mutant Gene mio16 of Maize Using Double Selected Amplification of Insertion Flanking Fragments (DSAIFF)

Mutator transposable element (Mu) has been used as an effective tool to clone maize (Zea mays L.) genes. One opaque endosperm mutant (mio16) was identified in a pool of Mu inserted mutants. A modified method, termed the double selected amplification of insertion flanking fragments (DSAIFF), was empl...

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Autors principals: Wen-juan ZHONG, Mei-dong ZHANG, Liu-qi YANG, Ming-chun WANG, Yong-lian ZHENG, Wen-peng YANG, You-jun GAO
Format: Artigo
Idioma:Inglês
Publicat: KeAi Communications Co., Ltd. 2012-10-01
Col·lecció:Journal of Integrative Agriculture
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Accés en línia:http://www.sciencedirect.com/science/article/pii/S2095311912601622
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