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γ-Secretase Epsilon-cleavage Assay

γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are HEK293 cells with a stably integrated luciferase reporter under...

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Principais autores: Ting-Hai Xu, Yan Yan, Kaleeckal Harikumar, Laurence Miller, Karsten Melcher, H. Xu
Formato: Artigo
Idioma:Inglês
Publicado: Bio-protocol LLC 2017-11-01
Series:Bio-Protocol
Acceso en liña:https://bio-protocol.org/en/bpdetail?id=2900&type=0
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