Purification of full-length recombinant human huntingtin proteins with allelic series of polyglutamine lengths

Summary: Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by the polyglutamine (polyQ) expansion in huntingtin (HTT) protein. The challenge of obtaining full-length HTT proteins with high purity limits the understanding of the HTT protein function. Here, we provid...

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Hlavní autoři:	Hyeongju Kim, Kyung-gi Hyun, Alejandro Lloret, Ihn Sik Seong, Ji-Joon Song
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2021-12-01
Edice:	STAR Protocols
Témata:	Protein Biochemistry Protein expression and purification Cryo-EM
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S266616672100592X
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