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Simultaneous Detection of RIG-1, MDA5, and IFIT-1 Expression Is a Convenient Tool for Evaluation of the Interferon-Mediated Response

In this study, we developed a novel, multiplex qPCR assay for simultaneous detection of RIG-1, MDA5, and IFIT-1 at the mRNA level. The assay was validated in A549 cells transfected with in vitro transcribed RNAs. Both exogenous RNA-GFP and self-amplifying (saRNA-GFP) induced significant expression o...

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Asıl Yazarlar: Alexey A. Lozhkov, Marina A. Plotnikova, Marya A. Egorova, Irina L. Baranovskaya, Ekaterina A. Elpaeva, Sergey A. Klotchenko, Andrey V. Vasin
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: MDPI AG 2022-09-01
Seri Bilgileri:Viruses
Konular:
qPCR
cytosolic sensors
interferons
ISGs
in vitro transcribed RNAs
influenza A virus
Online Erişim:https://www.mdpi.com/1999-4915/14/10/2090
Etiketler: Etiketle
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