Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation
A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on Percoll gradient step. The intactness and purity...
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| Principais autores: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Polish Botanical Society
2012-11-01
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| Serier: | Acta Societatis Botanicorum Poloniae |
| Fag: | |
| Online adgang: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/1233 |
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