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Culture and Nucleofection of Postnatal Day 7 Cortical and Cerebellar Mouse Astroglial Cells

Lineage reprogramming of astroglial cells isolated from different brain regions leads to the generation of different neuronal subtypes. This protocol describes the isolation and culture of neocortical and cerebellar astrocytes from postnatal mice. We also present a comprehensive description of the m...

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Autori principali: Malek Chouchane, Marcos Costa
Natura: Artigo
Lingua:Inglês
Pubblicazione: Bio-protocol LLC 2018-02-01
Serie:Bio-Protocol
Accesso online:https://bio-protocol.org/en/bpdetail?id=2712&type=0
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