Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells

Manual analysis of flow cytometry data and subjective gate-border decisions taken by individuals continue to be a source of variation in the assessment of antigen-specific T cells when comparing data across laboratories, and also over time in individual labs. Therefore, strategies to provide automat...

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Główni autorzy:	Natasja Wulff Pedersen, P. Anoop Chandran, Yu Qian, Jonathan Rebhahn, Nadia Viborg Petersen, Mathilde Dalsgaard Hoff, Scott White, Alexandra J. Lee, Rick Stanton, Charlotte Halgreen, Kivin Jakobsen, Tim Mosmann, Cécile Gouttefangeas, Cliburn Chan, Richard H. Scheuermann, Sine Reker Hadrup
Format:	Artigo
Język:	Inglês
Wydane:	Frontiers Media S.A. 2017-07-01
Seria:	Frontiers in Immunology
Hasła przedmiotowe:	major histocompatibility complex multimers antigen-specific T cells automated gating computational analysis major histocompatibility complex dextramers flow cytometry
Dostęp online:	http://journal.frontiersin.org/article/10.3389/fimmu.2017.00858/full
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