Protocol for High-Resolution Mapping of Splicing Products and Isoforms by RT-PCR Using Fluorescently Labeled Primers
Summary: We describe an RT-PCR protocol that allows high-resolution mapping of splicing products and isoforms using fluorescently labeled primers. Each species contains one fluorescent group allowing a direct comparison of the different isoforms despite size differences. A custom-size ladder enables...
Salvato in:
| Autori principali: | , , , , |
|---|---|
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2020-12-01
|
| Serie: | STAR Protocols |
| Accesso online: | http://www.sciencedirect.com/science/article/pii/S2666166720301271 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|