An optimized protocol to assess SUMOylation in the plant Capsella rubella using two-component DEX-inducible transformants
Summary: Here, we present an efficient protocol to test the SUMOylation of a target protein in the plant Capsella rubella based on overexpression of dexamethasone (DEX)-inducible tagged proteins. We describe the construction of two-component, FLAG-tagged DEX-inducible plasmids. We then detail the tr...
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| Autori principali: | , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2022-03-01
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| Serie: | STAR Protocols |
| Soggetti: | |
| Accesso online: | http://www.sciencedirect.com/science/article/pii/S2666166722000776 |
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