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Duplex digital PCR assay on microfluidic chamber arrays for total HIV DNA reservoir quantification in persons with HIV

Abstract HIV reservoir latently persists in people with HIV (PWH) despite long-term antiretroviral therapy (ART). Total HIV DNA quantification is commonly employed as a surrogate marker to assess the size of the viral reservoir. We developed a duplex digital PCR assay to easily quantify the total HI...

Ausführliche Beschreibung

Gespeichert in:
Hauptverfasser: Lucía Gutiérrez-García, Andrés Esteban-Cantos, Javier Rodríguez-Centeno, Cristina Marcelo-Calvo, Jose R. Arribas, Berta Rodés
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Portfolio 2025-08-01
Schriftenreihe:Scientific Reports
Schlagworte:
Digital PCR
HIV
Reservoir
Microchamber
Multiplex PCR
Online-Zugang:https://doi.org/10.1038/s41598-025-17392-5
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