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Benchmarking a targeted 16S ribosomal RNA gene enrichment approach to reconstruct ancient microbial communities

The taxonomic characterization of ancient microbiomes is a key step in the rapidly growing field of paleomicrobiology. While PCR amplification of the 16S ribosomal RNA (rRNA) gene is a widely used technique in modern microbiota studies, this method has systematic biases when applied to ancient micro...

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Principais autores: Raphael Eisenhofer, Sterling Wright, Laura Weyrich
Format: Artigo
Sprog:Inglês
Udgivet: PeerJ Inc. 2024-03-01
Serier:PeerJ
Fag:
Ancient DNA
Paleomicrobiology
Dental calculus
Oral microbiome
Shotgun metagenomics
Bioinformatics
Online adgang:https://peerj.com/articles/16770.pdf
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