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Preparation of a phosphotyrosine-protein standard for use in semiquantitative western blotting with enhanced chemiluminescence.

Protein tyrosine phosphorylation is key to activation of receptor tyrosine kinases (RTK) that drive development of some cancers. One challenge of RTK-targeted therapy is identification of those tumors that express non-mutated but activated RTKs. Phosphotyrosine (pTyr) RTK levels should be more predi...

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Autors principals: Nancy Kendrick, Ginny Powers, Jon Johansen, Matt Hoelter, Andrew Koll, Sofia Carlson, Devika Channaveerappa, Costel C Darie
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2020-01-01
Col·lecció:PLoS ONE
Accés en línia:https://doi.org/10.1371/journal.pone.0234645
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