Protocol for purification, crystallization, and structure determination of the mpox virus mRNA cap N7 methyltransferase complex

Summary: The mpox virus (MPXV) mRNA cap N7 methyltransferase (RNMT) methylates guanosine at mRNA 5′-cap N7 positions to enable immune evasion. Here, we present a protocol for E1CTD-E12 complex purification and crystallization. We describe steps for rational sequence design of the complex, co-express...

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Principais autores:	Qingqing Guo, Anke Chen, Jin Ding, Jixi Li
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2025-06-01
coleção:	STAR Protocols
Assuntos:	Molecular Biology Protein expression and purification Structural Biology X-ray Crystallography
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S2666166725002515
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