Solid Phase Assembly of Fully Protected Trinucleotide Building Blocks for Codon-Based Gene Synthesis

The use of pre-formed trinucleotides, representing codons of the 20 canonical amino acids, for oligonucleotide-directed mutagenesis offers the advantage of controlled randomization and generation of “smart libraries”. We here present a method for the preparation of fully protected trinuc...

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Hlavní autoři:	Ruth Suchsland, Bettina Appel, Matthäus Janczyk, Sabine Müller
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	MDPI AG 2019-05-01
Edice:	Applied Sciences
Témata:	trinucleotide building block gene library protein engineering codon randomization
On-line přístup:	https://www.mdpi.com/2076-3417/9/11/2199
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