Methanol feeding strategies for high-yield production of a collagen-based protein in Komagataella phaffii

Abstract The recombinant production of extracellular matrix proteins is a promising approach for replacing animal-derived materials in biomedical applications. K. phaffii represents a favorable expression host because it combines the ability of higher eukaryotes for secreted protein production with...

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Bibliografiske detaljer

Principais autores:	Jan Peter Ebbecke, Domenic Schlauch, Charlotte Güler, Hamidreza Pirmahboub, Selin Kara, Iliyana Pepelanova
Format:	Artigo
Sprog:	Inglês
Udgivet:	Springer 2025-12-01
Serier:	Applied Microbiology and Biotechnology
Fag:	Collagen Komagataella phaffii Recombinant protein expression Bioprocess optimization Methanol feeding strategy Stirred tank reactor
Online adgang:	https://doi.org/10.1007/s00253-025-13675-z
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