A CRISPR-Cas9-Mediated Large-Fragment Assembly Method for Cloning Genomes and Biosynthetic Gene Cluster

The ability to clone large DNA fragments from genomes is valuable for both basic and applied research, such as the construction of synthetic genomes, and the expression of biosynthetic gene clusters (BGCs) for natural product discovery. Here, we report a fast and efficient platform for the direct ca...

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Manylion Llyfryddiaeth

Prif Awduron:	Yujing Guo, Guang Cai, Huiying Li, Zhenquan Lin, Shuobo Shi, Jin Jin, Zihe Liu
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	MDPI AG 2024-07-01
Cyfres:	Microorganisms
Pynciau:	CRISPR/Cas9 <i>in vitro</i> DNA assembly synthetic genomes biosynthetic gene cluster
Mynediad Ar-lein:	https://www.mdpi.com/2076-2607/12/7/1462
Tagiau:	Ychwanegu Tag Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!