Characterization of the Impact of Density Gradient Centrifugation on the Profile of the Pig Sperm Transcriptome by RNA-Seq

RNA-Seq data from human semen suggests that the study of the sperm transcriptome requires the previous elimination from the ejaculates of somatic cells carrying a larger load of RNA. Semen purification is also carried to study the sperm transcriptome in other species including swine and it is often...

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Hauptverfasser:	Yu Lian, Marta Gòdia, Anna Castello, Joan Enric Rodriguez-Gil, Sam Balasch, Armand Sanchez, Alex Clop
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Frontiers Media S.A. 2021-07-01
Schriftenreihe:	Frontiers in Veterinary Science
Schlagworte:	sperm RNA RNA-Seq sperm purification differentially abundant gene somatic cell germline cell
Online-Zugang:	https://www.frontiersin.org/articles/10.3389/fvets.2021.668158/full
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