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Monitoring cell fate in 3D organotypic human squamous epithelial cultures

Summary: Here, we provide a protocol to model the effects of changes to a small number of cells, such as those arising from a mutation or a virus infection, in stratified epithelia. We describe steps for diluting engineered human keratinocytes into a larger population of unmodified cells and using t...

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Autori principali: Joshua Hatterschide, Christopher A. Natale, Todd W. Ridky, Elizabeth A. White
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2023-03-01
Serie:STAR Protocols
Soggetti:
Cell Biology
Cell Culture
Cancer
Microbiology
Cell Differentiation
Organoids
Accesso online:http://www.sciencedirect.com/science/article/pii/S266616672300059X
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