DNA Free CRISPR/DCAS9 Based Transcriptional Activation System for <i>UGT76G1</i> Gene in <i>Stevia rebaudiana</i> Bertoni Protoplasts
The UDP-glycosyltransferase 76G1 (<i>UGT76G1</i>) is responsible for the conversion of stevioside to rebaudioside A. Four single guide RNAs (sgRNAs) were designed from the <i>UGT76G1</i> proximal promoter region of stevia by using the online-based tool, benchling. The dCas9 fused with VP64 as a tran...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MDPI AG
2022-09-01
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| Serie: | Plants |
| Soggetti: | |
| Accesso online: | https://www.mdpi.com/2223-7747/11/18/2393 |
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