A simple method for non-denaturing purification of biotin-tagged proteins through competitive elution with free biotin
The use of avidin or streptavidin in the purification of biotinylated proteins has been highly restricted due to the harsh and denaturing elution conditions. Here, we use biotinylated bovine serum albumin as a working model to demonstrate a simple and rapid method for biotin-tagged protein purificat...
Uloženo v:
| Hlavní autoři: | , , , , , |
|---|---|
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Taylor & Francis Group
2020-01-01
|
| Edice: | BioTechniques |
| Témata: | |
| On-line přístup: | https://www.future-science.com/doi/10.2144/btn-2019-0088 |
| Tagy: |
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
|