Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases

Abstract The functional diversification of O2-tolerant [NiFe]-hydrogenases using orthogonal translation systems (OTSs) offers a promising strategy for developing advanced biocatalysts and biohybrid energy platforms. However, plasmid-based OTSs frequently impose metabolic burdens and suffer from plas...

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Autors principals:	Qin Fan, Stevanie Stevanie, Stefan Frielingsdorf, Peter Neubauer, Oliver Lenz, Matthias Gimpel
Format:	Artigo
Idioma:	Inglês
Publicat:	BMC 2026-07-01
Col·lecció:	Microbial Cell Factories
Matèries:	CRISPR/Cas9 [NiFe]-hydrogenase Cupriavidus necator Orthogonal translation system (OTS) Genomic integration Genetic code expansion
Accés en línia:	https://doi.org/10.1186/s12934-026-03057-z
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