Development and laboratory validation of duplex real-time PCR for simultaneous detection of Brucella and bovine alphaherpesvirus from clinical specimens

A duplex real‑time PCR was developed and validated for the simultaneous detection of Brucella and bovine alphaherpesvirus‑1 (BoHV‑1) from bovine clinical specimens. The bcsp31 gene of Brucella and gB gene of BoHV‑1 were used as targets in the assay. The limit of detection for BoHV‑1 was 0.03 TCID50...

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Prif Awduron:	Laxmi Narayan Saarangi, Supriya Polapally, Samir Kumar Rana, Vijay Shriram Bahekar, Kota Sri Naga Leela Surendra, Rachamreddy Venkata Chandrasekhar Reddy, Aparna Sri Raichur, Ponnanna Nadikerianda Muthappa, Girish Kumar Sharma
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale 2020-04-01
Cyfres:	Veterinaria Italiana
Pynciau:	Bovine abortion Bovine alphaherpesvirus Brucella Diagnosis Infectious bovine rhinotracheitis Multiplex Real Time PCR
Mynediad Ar-lein:	https://www.veterinariaitaliana.izs.it/index.php/VetIt/article/view/1728
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