HighALPS: ultra-high-throughput marker-gene amplicon library preparation and sequencing on the Illumina NextSeq and NovaSeq Platforms

ABSTRACT Microbiome research using amplicon sequencing of microbial marker genes has surged over the past decade, propelled by protocols for highly multiplexed sequencing with barcoded primer constructs. Newer Illumina platforms like the NovaSeq and NextSeq series significantly outperform older sequ...

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Prif Awduron:	Lena Flörl, Paula Momo Cabrera, Maria Domenica Moccia, Serafina Plüss, Nicholas A. Bokulich
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	American Society for Microbiology 2026-03-01
Cyfres:	mSystems
Pynciau:	amplicon library preparation microbiome protocol high-throughput sequencing 16S rRNA gene sequencing fungal ITS sequencing
Mynediad Ar-lein:	https://journals.asm.org/doi/10.1128/msystems.00023-26
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