High-resolution melting curve FRET-qPCR rapidly distinguishes Streptococcus equi subsp. equi and zooepidemicus

ABSTRACT We developed a fluorescence resonance energy transfer (FRET)-based real-time PCR (qPCR) assay coupled with high-resolution melting (HRM) analysis to differentiate Streptococcus equi subsp. equi (SEE) from S. equi subsp. zooepidemicus (SEZ), two closely related equine pathogens with distinct...

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Detalhes bibliográficos

Principais autores:	Nneka Vivian Iduu, Donna Raiford, Noah D. Cohen, Kerstin K. Landrock, Chengming Wang
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology 2025-09-01
coleção:	Microbiology Spectrum
Assuntos:	Streptococcus equi subsp. equi Streptococcus equi subsp. zooepidemicus strangles high-resolution melting curve analysis FRET-qPCR molecular diagnostics
Acesso em linha:	https://journals.asm.org/doi/10.1128/spectrum.01529-25
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