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Endonuclease specificity and sequence dependence of type IIS restriction enzymes.

Restriction enzymes that recognize specific sequences but cleave unknown sequence outside the recognition site are extensively utilized tools in molecular biology. Despite this, systematic functional categorization of cleavage performance has largely been lacking. We established a simple and automat...

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Autors principals: Sverker Lundin, Anders Jemt, Finn Terje-Hegge, Napoleon Foam, Erik Pettersson, Max Käller, Valtteri Wirta, Preben Lexow, Joakim Lundeberg
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science (PLoS) 2015-01-01
Col·lecció:PLoS ONE
Accés en línia:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0117059&type=printable
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