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The Vacc-SeqQC project: Benchmarking RNA-Seq for clinical vaccine studies

IntroductionOver the last decade, the field of systems vaccinology has emerged, in which high throughput transcriptomics and other omics assays are used to probe changes of the innate and adaptive immune system in response to vaccination. The goal of this study was to benchmark key technical and ana...

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Guardat en:
Autors principals: Johannes B. Goll, Steven E. Bosinger, Travis L. Jensen, Hasse Walum, Tyler Grimes, Gregory K. Tharp, Muktha S. Natrajan, Azra Blazevic, Richard D. Head, Casey E. Gelber, Kristen J. Steenbergen, Nirav B. Patel, Patrick Sanz, Nadine G. Rouphael, Evan J. Anderson, Mark J. Mulligan, Daniel F. Hoft
Format: Artigo
Idioma:Inglês
Publicat: Frontiers Media S.A. 2023-01-01
Col·lecció:Frontiers in Immunology
Matèries:
RNA-Seq
statistical power
ERCC
tularemia vaccine (DVC-LVS)
gene filtering
sequencing depth
Accés en línia:https://www.frontiersin.org/articles/10.3389/fimmu.2022.1093242/full
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