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Structure-based engineering of heparinase I with improved specific activity for degrading heparin

Abstract Background Heparinase I from Pedobacter heparinus (Ph-HepI), which specifically cleaves heparin and heparan sulfate, is one of the most extensively studied glycosaminoglycan lyases. Enzymatic degradation of heparin by heparin lyases not only largely facilitates heparin structural analysis b...

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Principais autores: Chuan Zhang, Bao-Cheng Yang, Wen-Ting Liu, Zhong-Yuan Li, Ya-Jian Song, Tong-Cun Zhang, Xue-Gang Luo
Format: Artigo
Sprog:Inglês
Udgivet: BMC 2019-08-01
Serier:BMC Biotechnology
Fag:
Heparinase I
Specific activity
Site-directed mutagenesis
Homology modeling
Molecular docking
Online adgang:http://link.springer.com/article/10.1186/s12896-019-0553-3
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