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High-performance gene expression and knockout tools using sleeping beauty transposon system

Abstract Background Similar to retro−/lenti- virus system, DNA transposons are useful tools for stable expression of exogenous genes in mammalian cells. Sleeping Beauty (SB) transposon has adopted for integrating genes into host genomes in recent studies. However, SB-derived vector system for protei...

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Main Authors: Kaishun Hu, Yu Li, Wenjing Wu, Hengxing Chen, Zhen Chen, Yin Zhang, Yabin Guo, Dong Yin
Format: Artigo
Language:Inglês
Published: BMC 2018-11-01
Series:Mobile DNA
Subjects:
Sleeping beauty
FBW7
CRISPR
NFATc1
RCC2
BRD7
Online Access:http://link.springer.com/article/10.1186/s13100-018-0139-y
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