β-Actin as an Endogenous Control Gene in Real-Time PCR for Detection of West Nile and Usutu Virus in Mosquitoes

Mosquito-borne viruses like West Nile virus (WNV) and Usutu virus (USUV) present growing public health concerns, especially with climate change and expanding vector ranges. This study describes the development and validation of a duplex Real-Time RT-PCR assay targeting β-actin (ACTB) mRNA as an endo...

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Principais autores:	Jeanne Lai, Carlotta Tessarolo, Elisabetta Ercole, Marina Gallo, Monica Lo Faro, Claudia Palmitessa, Valerio Carta, Alessio Ferrari, Alessandra Favole, Mattia Begovoeva, Francesco Ingravalle, Simone Peletto, Nicolò Francesco Fiscella, Roberta Irelli, Eugenia Ciarrocchi, Walter Martelli, Andrea Mosca, Giulia Cagnotti, Cristina Casalone, Cristiano Corona
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2025-10-01
coleção:	Microorganisms
Assuntos:	West Nile virus Usutu virus β-Actin (ACTB) mosquitoes borne virus surveillance internal control housekeeping gene
Acesso em linha:	https://www.mdpi.com/2076-2607/13/11/2518
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