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Protocol to develop a proximal tubule-on-chip model based on hiPSC-derived kidney organoids for functional analysis of renal transporters

Summary: Here, we present a protocol for describing the generation of a proximal tubule-on-chip model using human induced pluripotent stem cell (hiPSC)-derived kidney organoids for renal transporter analysis. We describe steps for hiPSC differentiation into kidney organoids, proximal tubule cell iso...

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Principais autores: Cheng Ma, Ramin Banan Sadeghian, Ryosuke Negoro, Kazuya Fujimoto, Toshikazu Araoka, Naoki Ishiguro, Minoru Takasato, Ryuji Yokokawa
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2025-06-01
Serier:STAR Protocols
Fag:
Stem Cells
Tissue Engineering
Biotechnology and bioengineering
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166725001832
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