Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples

Bluetongue (BT) is a major Office International des Epizooties (OIE)-listed disease of wild and domestic ruminants caused by several serotypes of Bluetongue virus (BTV), a virus with a segmented dsRNA genome belonging to the family Reoviridae, genus Orbivirus. BTV is transmitted through the bites of...

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Hlavní autoři:	Angela M. Rocchigiani, Maria G. Tilocca, Ottavio Portanti, Bruna Vodret, Roberto Bechere, Marco Di Domenico, Giovanni Savini, Alessio Lorusso, Giantonella Puggioni
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Frontiers Media S.A. 2020-04-01
Edice:	Frontiers in Veterinary Science
Témata:	Bluetongue Reoviridae RNA quantification droplet digital RT-PCR Quantitative Real Time -RT- PCR
On-line přístup:	https://www.frontiersin.org/article/10.3389/fvets.2020.00170/full
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