A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido

INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were u...

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Auteurs principaux:	Andrea Cristine Koishi, Débora Fonseca Vituri, Pedro Sebastião Raimundo Dionízio Filho, Alexandre Augusto Sasaki, Maria Sueli Soares Felipe, Emerson José Venancio
Format:	Artigo
Langue:	Inglês
Publié:	Sociedade Brasileira de Medicina Tropical (SBMT) 2010-12-01
Collection:	Revista da Sociedade Brasileira de Medicina Tropical
Sujets:	Paracoccidioides brasiliensis Semi-nested PCR Detecção molecular Molecular detection
Accès en ligne:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000600026
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