Effect of DNA sequence of Fab fragment on yield characteristics and cell growth of E. coli
Abstract Codon usage is one of the factors influencing recombinant protein expression. We were interested in the codon usage of an antibody Fab fragment gene exhibiting extreme toxicity in the E. coli host. The toxic synthetic human Fab gene contained domains optimized by the “one amino acid-one cod...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Portfolio
2017-06-01
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| Col·lecció: | Scientific Reports |
| Accés en línia: | https://doi.org/10.1038/s41598-017-03957-6 |
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