Discovery and Characterization of an ALFA-Tag-Specific Affinity Resin Optimized for Protein Purification at Low Temperatures in Physiological Buffer

Epitope tags are widely employed as tools to detect, purify and manipulate proteins in various experimental systems. We recently introduced the ALFA-tag together with two ALFA-specific single-domain antibodies (sdAbs), NbALFA and NbALFA^PE, featuring high or intermediate affinity, respecti...

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Detalhes bibliográficos

Principais autores:	Markus Kilisch, Hansjörg Götzke, Maja Gere-Becker, Alexander Crauel, Felipe Opazo, Steffen Frey
Formato:	Artigo
Idioma:	Inglês
Publicado em:	MDPI AG 2021-02-01
Colecção:	Biomolecules
Assuntos:	nanobody sdAbs epitope tag affinity immunoprecipitation native elution
Acesso em linha:	https://www.mdpi.com/2218-273X/11/2/269
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