Exclusive Amplification of cDNA Template (EXACT) RT-PCR to Avoid Amplifying Contaminating Genomic Pseudogenes
Genomic DNA contamination within RNA samples has important implications for RT-PCR, particularly if there is a pseudogene related to the gene under investigation, because amplification from pseudogenes and reverse-transcribed cDNA can be very difficult to distinguish. Methods to remove DNA contamina...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
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Taylor & Francis Group
2001-10-01
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| Schriftenreihe: | BioTechniques |
| Online-Zugang: | https://www.future-science.com/doi/10.2144/01314st03 |
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