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Diagnostic value of metagenomic next-generation sequencing in detecting Pneumocystis jirovecii pneumonia in HIV-infected patients

IntroductionAccurate diagnosis of Pneumocystis jirovecii pneumonia (PJP) in HIV patients remains challenging. This study compares metagenomic next-generation sequencing (mNGS) with PCR, GMS staining, and serum β-D-glucan (BG) assays for PJP detection and co-infection identification.MethodsBALF sampl...

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Main Authors: Jiawen He, Ying Chen, Zhuxiu Jiang, Feng Li, Mingli Zhu, Zhibo Xu, Meihua Wang, Meng Tang, Yuanting Wu, Yang Li
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2025-03-01
Series:Frontiers in Medicine
Subjects:
metagenomic next-generation sequencing
HIV
Pneumocystis jirovecii pneumonia
bronchoalveolar lavage fluid
diagnostic performance
co-infection
Online Access:https://www.frontiersin.org/articles/10.3389/fmed.2025.1567484/full
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