cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake

To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus coll...

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Bibliografski detalji

Glavni autori:	F.H.R. Fagundes, M. Oliveira, S. Huancahuire-Vega, F.F. Romero-Vargas, L.A. Ponce-Soto, S. Marangoni
Format:	Artigo
Jezik:	Inglês
Izdano:	Associação Brasileira de Divulgação Científica 2010-03-01
Serija:	Brazilian Journal of Medical and Biological Research
Teme:	Cdc PLA2 cDNA Chick biventer cervicis Crotalus durissus collilineatus Mass spectrometry MALDI-TOF Neurotoxic agents
Online pristup:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300007
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