Evaluation of the influence of RNA extraction methods on the detection of citrus viruses and viroids

Abstract Background Certification and disease management practices are reliant on accurate pathogen detection assays. Effective nucleic acid extraction is the cornerstone of various molecular detection techniques, and many virus and viroid RNA extraction protocols have been developed, each with acco...

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Principais autores:	Madelein Dippenaar, Hans Jacob Maree, Rachelle Bester
Formato:	Artigo
Idioma:	Inglês
Publicado em:	BMC 2025-12-01
coleção:	Virology Journal
Assuntos:	Virus discovery Illumina RT-qPCR High-throughput sequencing RNA viruses Pathogen identification
Acesso em linha:	https://doi.org/10.1186/s12985-025-03013-0
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