Development of cell-based assay for detecting replication-competent adeno-associated virus by qPCR

Recombinant, non-replicative adeno-associated virus (AAV) containing a therapeutic gene of interest (GOI) is widely used as a vector for gene therapy. One way to manufacture AAV is through triple-transfection of HEK293 cells, with plasmids containing the (1) GOI, (2) replication (rep2) and capsid (c...

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Hlavní autoři:	Aishwarya Shevade, John S. Reeves, Hanne Bak, Andrew D. Tustian
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Elsevier 2025-09-01
Edice:	Molecular Therapy: Methods & Clinical Development
Témata:	adeno-associated viral vector AAV replication-competent AAV quality control critical quality attribute rcAAV
On-line přístup:	http://www.sciencedirect.com/science/article/pii/S232905012500124X
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