The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers.

BACKGROUND: Due to the inherent sensitivity of human embryonic stem cells (hESCs) to manipulations, the recovery and survival of hESCs after fluorescence-activated cell sorting (FACS) can be low. Additionally, a well characterized and robust methodology for performing FACS on hESCs using multiple-ce...

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Detalhes bibliográficos

Principais autores:	Nil Emre, Jason G Vidal, Jeanne Elia, Eric D O'Connor, Rosanto I Paramban, Michael P Hefferan, Roman Navarro, Danielle S Goldberg, Nissi M Varki, Martin Marsala, Christian T Carson
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Public Library of Science (PLoS) 2010-01-01
coleção:	PLoS ONE
Acesso em linha:	http://europepmc.org/articles/PMC2921395?pdf=render
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