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Separation of bivalent anti-T cell immunotoxin from Pichia pastoris glycoproteins by borate anion exchange

A major problem encountered in the large-scale purification of the bivalent anti-T cell immunotoxin, A-dmDT390-bisFv(G4S), from Pichia pastoris supernatants was the presence of host glycoproteins exhibiting similar charge, size, and hydrophobicity characteristics. We overcame this problem by employi...

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Príomhchruthaitheoirí: Jung Hee Woo, David M. Neville
Formáid: Artigo
Teanga:Inglês
Foilsithe / Cruthaithe: Taylor & Francis Group 2003-08-01
Sraith:BioTechniques
Rochtain ar líne:https://www.future-science.com/doi/10.2144/03352pt04
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