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One‐step selective affinity purification and immobilization of His‐tagged enzyme by recyclable magnetic nanoparticles

Abstract The NiFe2O4 magnetic nanoparticles (NF‐MNPs) were prepared for one‐step selective affinity purification and immobilization of His‐tagged recombinant glucose dehydrogenase (GluDH). The prepared nanoparticles were characterized by a Fourier‐transform infrared spectrophotometer and microscopy....

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Библиографические подробности
Главные авторы: Li‐Jian Zhou, Rui‐Fang Li, Xue‐Yong Li, Ye‐Wang Zhang
Формат: Artigo
Язык:Inglês
Опубликовано: Maximum Academic Press 2021-06-01
Серии:Engineering in Life Sciences
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Online-ссылка:https://doi.org/10.1002/elsc.202000093
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