Cosolvent-induced spontaneous refolding of lipase
Abstract The overexpression of proteins in Escherichia coli often results in the formation of inclusion bodies, which are biologically inactive, especially for proteins with exposed hydrophobic surfaces. Solubilization of inclusion bodies (IBs) and subsequent refolding is essential for obtaining cor...
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| Hauptverfasser: | , , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Springer
2026-01-01
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| Schriftenreihe: | Applied Microbiology and Biotechnology |
| Schlagworte: | |
| Online-Zugang: | https://doi.org/10.1007/s00253-025-13660-6 |
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