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Expression and purification of recombinant Lactobacillus casei bacteriocin and analysis of its antibacterial activity

Diseases caused by foodborne pathogens have largely promoted the search for natural biopreservatives. We constructed recombinant plasmids pGEX-4 T-1-lacA, pGEX-4 T-1-lacB, and pGEX-4 T-1-lacAB, harboring genes for bacteriocins lactocin (Lac) A, B, and AB, respectively, with protein expression achiev...

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Main Authors: Wei Yu, Jiage Ma, Xiaoqian Chen, Ying Tan, Pin Chen, Xumeng Zhu, Libo Liu
Format: Artigo
Language:Inglês
Published: Taylor & Francis Group 2020-01-01
Series:CyTA - Journal of Food
Subjects:
bacteriocin
lactobacillus casei
heterologous expression
antimicrobial activity
protein purification
Online Access:http://dx.doi.org/10.1080/19476337.2020.1749134
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