Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression
The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Portfolio
2019-04-01
|
| Schriftenreihe: | Nature Communications |
| Online-Zugang: | https://doi.org/10.1038/s41467-019-09148-3 |
| Tags: |
Keine Tags, Fügen Sie das erste Tag hinzu!
|