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A Streamlined In Vitro mRNA Production Evaluation for mRNA-Based Vaccines and Therapeutics

To develop an effective strategy for in vitro mRNA production, it is crucial to evaluate the efficiency of the in vitro transcription platform. This can be accomplished using reporter genes, such as the luciferase-encoding gene. Luciferase activity assays provide a reliable means to assess the trans...

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Principais autores: Vittorio Madia, Sergio Minesso, Valentina Franceschi, Gaetano Donofrio
Format: Artigo
Sprog:Inglês
Udgivet: MDPI AG 2025-12-01
Serier:Methods and Protocols
Fag:
Luciferin
IRES
encephalomyocarditis virus
cap analog
mRNA
Online adgang:https://www.mdpi.com/2409-9279/8/6/153
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