Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader

Native interactions between lysophospholipids (LPs) and their cognate LP receptors are difficult to measure because of lipophilicity and/or the adhesive properties of lipids, which contribute to high levels of nonspecific binding in cell membrane preparations. Here, we report development of a free-s...

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Principais autores:	Manisha Ray, Kazufumi Nagai, Yasuyuki Kihara, Amanda Kussrow, Michael N. Kammer, Aaron Frantz, Darryl J. Bornhop, Jerold Chun
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Elsevier 2020-08-01
coleção:	Journal of Lipid Research
Assuntos:	receptor binding assay G protein-coupled receptor lysophospholipids molecular interaction interferometry free-solution assay-compensated interferometric reader
Acesso em linha:	http://www.sciencedirect.com/science/article/pii/S0022227520434923
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