Protocol for in vivo CRISPR screening targeting murine testicular cells
Summary: In vivo genome-wide screening elucidates tissue-specific molecular events. Here, we present a protocol for an in vivo genome-wide CRISPR-Cas9 single-guide RNA (sgRNA) library screening technique optimized for mouse testicular cells to investigate spermatogenesis. We describe steps for virus...
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| Principais autores: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2024-09-01
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| Serier: | STAR Protocols |
| Fag: | |
| Online adgang: | http://www.sciencedirect.com/science/article/pii/S2666166724004714 |
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