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Monitoring BCR-ABL transcript levels by real-time quantitative polymerase chain reaction: a linear regression equation to convert from BCR-ABL/B2M ratio to estimated BCR-ABL/ABL ratio

In order to overcome the problem of different control genes for BCR-ABL normalization, we used a linear regression equation to compare our results previously obtained using B2M as the control gene with those calculated using the ABL gene and validated the slope as a factor to convert from B2M to ABL...

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Autori principali: Ilaria Iacobucci, Livia Galletti, Marilina Amabile, Simona Soverini, Michele Baccarani, Giovanni Martinelli
Natura: Artigo
Lingua:Inglês
Pubblicazione: Ferrata Storti Foundation 2007-03-01
Serie:Haematologica
Accesso online:https://haematologica.org/article/view/4384
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